Quantitative Trait Loci Affecting Body Weight and Fatness From a Mouse Line Selected for Extreme High Growth

Author:

Brockmann Gudrun A1,Haley Chris S2,Renne Ulla1,Knott Sara A3,Schwerin Manfred1

Affiliation:

1. Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany

2. Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, United Kingdom

3. Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom

Abstract

Abstract Quantitative trait loci (QTL) influencing body weight were mapped by linkage analysis in crosses between a high body weight selected line (DU6) and a control line (DUKs). The two mouse lines differ in body weight by 106% and in abdominal fat weight by 100% at 42 days. They were generated from the same base population and maintained as outbred colonies. Determination of line-specific allele frequencies at microsatellite markers spanning the genome indicated significant changes between the lines on 15 autosomes and the X chromosome. To confirm these effects, a QTL analysis was performed using structured F2 pedigrees derived from crosses of a single male from DU6 with a female from DUKs. QTL significant at the genome-wide level were mapped for body weight on chromosome 11; for abdominal fat weight on chromosomes 4, 11, and 13; for abdominal fat percentage on chromosomes 3 and 4; and for the weights of liver on chromosomes 4 and 11, of kidney on chromosomes 2 and 9, and of spleen on chromosome 11. The strong effect on body weight of the QTL on chromosome 11 was confirmed in three independent pedigrees. The effect was additive and independent of sex, accounting for 21–35% of the phenotypic variance of body weight within the corresponding F2 populations. The test for multiple QTL onchromosome 11 with combined data from all pedigrees indicated the segregation of two loci separated by 36 cM influencing body weight.

Publisher

Oxford University Press (OUP)

Subject

Genetics

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4. Selection for body weight at 42 days in laboratory mice with and without litter size standardization—direct response and correlated effects on litter size;Bünger;Arch. Tierz.,1992

5. Quantitative trait loci for murine growth;Cheverud;Genetics,1996

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