INTERALLELIC COMPLEMENTATION AT THE sh LOCUS IN MAIZE AT THE ENZYME LEVEL

Abstract

ABSTRACT EMS-induced sh mutants and their heterozygotes were examined for the enzyme, sucrose synthetase, which has previously been shown to be coded by the Sh locus. Complementing heterozygotes have a wild-type phenotype, but show no hybrid protein band after starch gel electrophoresis. The existence of a heteromeric complex, however, is inferred from the two-fold elevation in sucrose cleavage activity in the complementing heterozygotes as compared to the mutant homozygotes. The observations on complementation described here are unique, as the elevation in the activity of this reversible enzyme is noticed only in one direction (viz, sucrose cleavage) of the reaction and not the other (sucrose synthesis).