Affiliation:
1. Vector Diagnostics Department, Walter Reed Army Institute of Research, Silver Spring, MD
2. Department of Entomology, The Pennsylvania State University, University Park, PA
Abstract
Abstract
Ticks can vector and transmit many pathogens and pose a serious human health threat throughout the world. After collection, many diagnostic laboratories must mechanically disrupt tick specimens for diagnostic testing and research purposes, but few studies have evaluated how well-commercial tissue homogenizers perform this task. We evaluated four commercially available tissue homogenizers: The Bead Ruptor 24 Elite, the Bullet Blender Storm, the gentleMACS Dissociator, and the Precellys 24. We quantitatively compared maceration level, nucleic acid quality, quantity, amplification, and DNA shearing to determine which machines performed the best. The Bead Ruptor 24 Elite had the highest overall score when disrupting a single, uninfected adult Amblyomma americanum (Linnaeus) (Ixodida: Ixodidae) and performed well in follow-on tests including disrupting individual juvenile samples and detecting pathogens from infected samples.
Funder
Department of Defense
Defense Health Program
Walter Reed Army Institute of Research
Publisher
Oxford University Press (OUP)
Subject
Infectious Diseases,Insect Science,General Veterinary,Parasitology
Cited by
4 articles.
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