Dietary Effects on the Development of Calliphora dubia and Chrysomya rufifacies (Diptera: Calliphoridae): Implications for Postmortem Interval

Abstract

Abstract Forensic entomology relies on insect development data generated within a laboratory setting in the estimation of minimum postmortem interval (mPMI). The methodologies used to produce these data vary considerably within the field and there is no accepted standard approach to laboratory rearing of forensically relevant species. A wide range of rearing media are used across published studies, including different species of animal and types of tissue (e.g., muscle and liver). Differing methodologies, particularly rearing diet, can introduce considerable variation into the baseline data upon which forensic estimates of the mPMI are calculated. Consequently, research establishing a widely available, standard and/or optimal, rearing medium for blow fly development for forensic application is desirable. This study examined dietary effects on the development of two forensically relevant blow fly species: Calliphora dubia Macquart, 1855, and Chrysomya rufifacies Macquart 1842 (Diptera: Calliphoridae). Larvae of both species were reared on pork liver, pork mince, pork loin, beef liver, beef mince, and guinea pig carcass under two constant temperature regimes (24 ± 1°C and 30 ± 1°C; 70 ± 10% humidity; 12-h/12-h photoperiod) to assess the influence of temperature on developmental response to diet. Fundamental developmental data pertaining to both species are reported. Developmental response to diet was species-specific and influenced by temperature with indication that the optimal temperature for C. dubia development is below 30°C. Pork mince was the most appropriate dietary standard of the rearing media investigated for the formulation of forensic development data for both species investigated.