Affiliation:
1. Universidad Complutense, Facultad de Veterinaria, Departamento de Nutrición y Bromatología III, 28040 Madrid, Spain
Abstract
Abstract
Raw and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the p53 gene. DNA from S. salar and O. mykiss was amplified by using primers flanking exons 5 to 6 of the p53 nuclear gene. PCR products of different length were obtained for each species (532 and 518 base pairs, respectively). Sequences of PCR products obtained from S. salar and O. mykiss were compared in the search for polymorphic restriction sites. The restriction fragments obtained with Eco RV, Hinf I, and Taq I endonucleases showed interspecific polymorphism, making it a useful method for identification of Atlantic salmon and rainbow trout.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
24 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献