Validation of an Immunoassay for Detection and Quantitation of a Genetically Modified Soybean in Food and Food Fractions Using Reference Materials: Interlaboratory Study
Author:
Lipp Markus1, Anklam Elke1, Stave James W2, Bahrs-Windsberger J, Barreto Crespo M T, Benvenuto E, Berben G, Brignon P, Busch U, Cordes C, Eklund E, El Jaziri M, Engel K H, Eriksen F, Eriksson S, Felinger A, Fogher C, Folch I, Garrett S, Hischenhuber C, Hörtner H, Jany K D, Krska R, Kruse L, Kuiper H A, Lobre C, Miraglia M, Mhller W, Nunes Costa J M, Oliveira L, Patel P, Pietsch K, Pöpping B, Remler P, Rentsch J, Schulze M, Sutton M, van Duijn G, van Kan F, Wurz A,
Affiliation:
1. European Commission, Joint Research Center, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit, T.P. 260, I-21020 Ispra (Va), Italy 2. Strategic Diagnostics, Inc., 111 Pencader Dr, Newark, DE 19702-3322
Abstract
Abstract
An immunoassay for detection of a specific genetically modified soybean (Roundup-Ready®) was validated on dried soybean powder in an interlaboratory study. Different percentages of genetically modified soybeans in nonmodified soybean matrix were evaluated in a blind study. Thirty-eight laboratories from 13 countries participated. The immunoassay was evaluated for 2 endpoints: (1) To give a semiquantitative result, i.e., determination of a given sample above or below a given threshold, or (2) to compute a quantitative result, i.e., percentage of genetically modified soybeans in the sample. Semiquantitative results showed that a given sample which contained <2% genetically modified soybeans was identified as below 2% with a 99% confidence level. Quantitative use of the assay resulted in a repeatability (r) and reproducibility (R) that were computed to be RSDr = 7% and RSDR = 10%, respectively, for a sample containing 2% genetically modified soybeans. Application of this method depends on availability of appropriate reference materials for a specific food matrix. Only matrix-matched reference materials can be used for analysis of food or food fractions.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
60 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Detection of Genetically Modified Additives in Meat Products in Riyadh City;Current Research in Nutrition and Food Science Journal;2022-04-29 2. Analytical strategy and interpretation of results;Application of Sampling and Detection Methods in Agricultural Plant Biotechnology;2022 3. Safety Issues of New Biotechnologies on Microbial, Animal, and Plant Cells;Advanced Fermentation and Cell Technology;2021-10-15 4. Food traceability;Innovative Food Analysis;2021 5. Enzyme-Linked Immunosorbent Assay (ELISA) Technique for Food Analysis;Techniques to Measure Food Safety and Quality;2021
|
|