Determination of Sulfonamides in Swine Meat by Immunoaffinity Chromatography

Abstract

Abstract A procedure was developed for the preparation of anti-sulfonamide (SA) group-specific antibodies and immunosorbents. Sulfonamide haptens and conjugates were synthesized by building spacer arms on an N1 group of 4-aminobenzensulfonamide. The anti-SA group-specific antibodies and immunosorbents were prepared successfully. After extraction with methanol–water (8 + 2), sulfamonomethoxine, sulfadimethoxine, and sulfaquinoxaline were cleaned up on immunoaffinity columns and determined by reversed-phase liquid chromatography with UV detection at 270 nm. The recoveries from fortified swine meat (10–100 μg/kg) ranged from 70.8 to 94.1%, with coefficients of variation of 3.4–12.9%. Limits of detection were 1–2 μg/kg.