Detection of Rift Valley Fever Virus Lineage H from South Africa through Syndromic Sentinel Surveillance Network in Senegal

Abstract

Abstract Background Rift Valley fever virus (RVFV) is an arbovirus that causes epizootics and epidemics among livestock population and humans. Our surveillance system has revealed multiple emergences and re-emergences of RVFV in West Africa over the last decade. Methods In Senegal a sentinel syndromic surveillance network (4S) has been implemented since 2011. Samples from human suspected arbovirus infection in 4S sentinel sites were sent to Institut Pasteur de Dakar (IPD) where arbovirus diagnosis by enzyme-linked immunosorbent assay (ELISA), real-time reverse transcription polymerase chain reaction (RT-PCR), and virus isolation were performed. Overall, IPD has received a total of 1,149 samples from arboviral suspected patients through the 4S network from January to December 2020. These samples were screened for seven arboviruses including RVFV. Whole genome sequencing of positive RVFV samples by RT-PCR were performed using Illumina Miseq platform followed by genome assembly. Phylogenetic analysis were performed using MEGA X. Results Out of the 1,149 arbovirus suspected cases, four RVFV positive samples were detected with RT-PCR while five RVFV positive samples were detected by ELISA. Complete genome sequences were obtained for three strains among the four positive samples by RT-PCR. Phylogenetic analyses indicated an emergence of a virus first described in South Africa during a major outbreak. Conclusion Strong surveillance system allowed the detection of RVFV outbreak in Senegal in 2020. The obtained genomes clustered with strains from South Africa belonging to lineage H. This calls for an implementation of a strong surveillance system in wild animals, humans, and livestock simultaneously in all African Countrries.