Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis-Reference-Cited by-同舟云学术

Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis

Published:2022-08-23 Issue:9 Volume:9 Page:
ISSN:2328-8957
Container-title:Open Forum Infectious Diseases
language:en
Short-container-title:

Author:

Young Bernadette C¹²^ORCID,Bush Stephen J¹,Lipworth Sam¹,George Sophie¹,Dingle Kate E¹,Sanderson Nick¹,Brankin Alice¹,Walker Timothy³,Sharma Srilakshmi⁴,Leong James⁴,Plaha Puneet⁵,Hofer Monika⁶,Chiodini Peter⁷,Gottstein B⁸,Furrer Lavinia⁸,Crook Derrick¹²⁹,Brent Andrew¹²

Affiliation:

1. Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital , Oxford , United Kingdom

2. Microbiology and Infectious Diseases Department, Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital , Oxford , United Kingdom

3. Oxford University Clinical Research Unit , Ho Chi Minh City , Vietnam

4. Oxford Eye Hospital, Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital , Oxford , United Kingdom

5. Nuffield Department of Surgical Sciences, University of Oxford, John Radcliffe Hospital , Oxford , United Kingdom

6. Department of Neuropathology, Oxford University Hospitals NHS Foundation Trust, John Radcliffe Hospital , Oxford , United Kingdom

7. Hospital of Tropical Diseases and the London School of Hygiene and Tropical Medicine London , London , United Kingdom

8. Institute for Infectious Diseases, University of Bern , Bern , Switzerland

9. National Institute for Health Research Oxford Biomedical Research Centre , Oxford , United Kingdom

Abstract

Abstract Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts.

Funder

National Institute for Health Research

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Oncology

Link

https://academic.oup.com/ofid/advance-article-pdf/doi/10.1093/ofid/ofac428/45497549/ofac428.pdf

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4. A dual PCR-based sequencing approach for the identification and discrimination of Echinococcus and Taenia taxa;Boubaker;Mol Cell Probes,2016