At a time when Europe and business stand at crossroads, this study provides a perspective into how business representation in the EU has evolved and valuable insights into how to organize lobbying strategies and influence policy-making. Uniquely, the study analyses business lobbying in Brussels by drawing on insights from political science, public management, and business studies. At the macro-level, we explore over thirty years of increasing business lobbying and explore the emergence of a distinct European business-government relations style. At the meso-level, we assess how the role of EU institutions, policy types, and the policy cycle shape the density and diversity of business activity. Finally, at the micro-level we seek to explore how firms organize their political affairs functions and mobilized strategic political responses. The study utilizes a variety of methods to analyse business-government relations drawing on unique company and policy-maker surveys; in-depth case studies and elite interviews; large statistical analysis of lobbying registers to examine business the density and diversity; and managerial career path and organizational analyses to assess corporate political capabilities. In doing so, this study contributes to discussions on corporate political strategy and interest groups activity. This monograph should be of interest to public policy scholars, policy-makers, and businesses managers seeking to understand EU government affair and political representation.