The objective of this book is to review the fundamental knowledge in ecology and evolution of infectious disease, as well as how it can be translated into opportunities to improve public health strategies in low-income countries, as well as in accounting and prompting for multisector interactions with the fields of economics, sociology and geopolitics, among others. The chapter explores challenges to applying the scientific knowledge for disease control, and it considers which disease to target. The chapter goes over the main recommendations arising from evolutionary ecology for infectious disease–control programs in low-income settings, and it highlights some crucial points that need to be considered to facilitate and accelerate integration of this fundamental knowledge to improve the efficiency of pathogen control.