A Two-color Single-molecule Sequencing Platform and Its Clinical Applications
Author:
Chen Fang1ORCID, Liu Bin1ORCID, Chen Meirong1ORCID, Jiang Zefei1ORCID, Zhou Zhiliang1ORCID, Wu Ping1ORCID, Zhang Meng1ORCID, Jin Huan1ORCID, Li Linsen1ORCID, Lu Liuyan1ORCID, Shang Huan1ORCID, Liu Lei1ORCID, Chen Weiyue1ORCID, Xu Jianfeng1ORCID, Sun Ruitao1ORCID, Wang Guangming1ORCID, Zheng Jiao1ORCID, Qi Jifang1ORCID, Yang Bo1ORCID, Zeng Lidong1ORCID, Li Yan1ORCID, Lv Hui1ORCID, Zhao Nannan1ORCID, Wang Wen1ORCID, Cai Jinsen1ORCID, Liu Yongfeng1ORCID, Luo Weiwei1ORCID, Zhang Juan1ORCID, Zhang Yanhua1ORCID, Fan Jicai1ORCID, Dan Haitao1ORCID, He Xuesen1ORCID, Huang Wei1ORCID, Sun Lei1ORCID, Yan Qin1ORCID
Affiliation:
1. GeneMind Biosciences Co., Ltd. , Shenzhen 518000, China
Abstract
Abstract
DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years. In this study, we developed a single-molecule desktop sequencer, GenoCare 1600 (GenoCare), which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry, making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use. Using the GenoCare platform, we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%. We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019 (COVID-19) samples from throat swabs. Our findings indicate that the GenoCare platform allows for microbial quantitation, sensitive identification of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, and accurate detection of virus mutations, as confirmed by Sanger sequencing, demonstrating its remarkable potential in clinical application.
Publisher
Oxford University Press (OUP)
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