Effects of whole corn high-grain diet feeding on ruminal bacterial community and epithelial gene expression related to VFA absorption and metabolism in fattening lambs

Abstract

Abstract The objective of this study was to investigate the effects of whole corn high-grain diet feeding on growth performance, ruminal bacterial community, and epithelial morphology and gene expression related to VFA absorption and metabolism in fattening lambs. Fourteen male (castrated) lambs were randomly assigned to either a group fed a ground corn high-grain diet (50.4% grain; HGC, n = 7) or a group fed a whole corn high-grain diet (50.4% grain; HWC, n = 7). After 7 wk of feeding, HWC group increased the average daily gain (ADG) (P = 0.036) and decreased the feed: gain value (P = 0.010) significantly. HWC group had a greater crude protein apparent digestibility (P = 0.028) in the third week and dry matter and neutral detergent fiber apparent digestibility (P < 0.05) in the seventh week. Pyrosequencing of the 16S ribosomal RNA gene revealed that HWC feeding increased the relative abundance of genera Anaerovibrio, Schwartzia and Unclassified Veillonellaceae in the rumen content and Howardella, Schwartzia and Unclassified Veillonellaceae in the rumen epithelia (P<0.05), while decreased the proportion of Lachnospira and Unclassified Synergistaceae in the rumen content and Anaerovorax, Papillibacter, Ruminococcus, Fibrobacter, Unclassified Lachnospiraceae, Unclassified Bacteroidales and Unclassified Prevotellaceae in the rumen epithelia (P < 0.05). HWC group increased the rumen papilla length (P = 0.001) and surface area (P = 0.002). Furthermore, HWC diet feeding up-regulated the relative mRNA expression of putative anion transporter isoform 1 (PAT1) (P = 0.032) in the rumen epithelia. In summary, compared with ground corn high-grain diet feeding, whole corn high-grain diet feeding improved animal performance, changed ruminal bacterial composition and diversity, and increased VFA absorption of epithelial papilla in fattening lambs. These findings provided theoretical guidance for the actual application of whole corn high-grain diet in ruminants.