Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples-Reference-Cited by-同舟云学术

Local surface plasmon resonance of gold nanoparticles as a correlative light and electron microscopy (CLEM) tag for biological samples

Published:2019-10-28 Issue: Volume: Page:
ISSN:2050-5698
Container-title:Microscopy
language:en
Short-container-title:

Author:

Haruta Tomohiro¹,Hasumi Keiko¹,Ikeda Yuta¹,Konyuba Yuji¹,Fukuda Tomohisa¹,Nishioka Hideo¹

Affiliation:

1. Application Management Department, JEOL Ltd, 3-1-2 Musashino, Akishima, Tokyo, Japan

Abstract

Abstract In this study, we investigated use of local surface plasmon resonance (LSPR) of metal nanoparticles (NPs) as a correlative light and electron microscopy (CLEM) tag for biological samples. Gold NPs in ultra-thin sections for TEM revealed that LSPR could be observed by optical microscopy at sizes of 20 nm or larger. Gold NPs at sizes less than 20 nm could be observed using the gold enhancement method. Therefore, this CLEM tag could be applied to immunoelectron microscopy using this gold enhancement method.

Publisher

Oxford University Press (OUP)

Subject

Radiology Nuclear Medicine and imaging,Instrumentation,Structural Biology

Link

http://academic.oup.com/jmicro/advance-article-pdf/doi/10.1093/jmicro/dfz031/30314894/dfz031.pdf

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