Affiliation:
1. Laboratory of Ultrastructural Virology, Institute for Frontier Life and Medical Sciences, Kyoto University, Japan
2. Institut für Virologie, Philipps-Universität Marburg, Germany
Abstract
Abstract
Lassa virus (LASV), belonging to the family Arenaviridae, causes severe haemorrhagic manifestations and is associated with a high mortality rate in humans. Thus, it is classified as a biosafety level (BSL)-4 agent. Since countermeasures for LASV diseases are yet to be developed, it is important to elucidate the molecular mechanisms underlying the life cycle of the virus, including its viral and host cellular protein interactions. These underlying molecular mechanisms may serve as the key for developing novel therapeutic options. Lymphocytic choriomeningitis virus (LCMV), a close relative of LASV, is usually asymptomatic and is categorized as a BSL-2 agent. In the present study, we visualized the transport of viral matrix Z protein in LCMV-infected cells using live-cell imaging microscopy. We demonstrated that the transport of Z protein is mediated by polymerized microtubules. Interestingly, the transport of LASV Z protein showed characteristics similar to those of Z protein in LCMV-infected cells. The live-cell imaging system using LCMV provides an attractive surrogate measure for studying arenavirus matrix protein transport in BSL-2 laboratories. In addition, it could be also utilized to analyze the interactions between viral matrix proteins and the cellular cytoskeleton, as well as to evaluate the antiviral compounds that target the transport of viral matrix proteins.
Funder
Japan Society for the Promotion of Science JSPS
AMED Research Program on Emerging and Re-emerging Infectious Diseases
AMED Japanese Initiative for Progress of Research on Infectious Disease for Global Epidemic
JSPS Core-to-Core Program A, the Advanced Research Networks
Grant for Joint Research Project of the Institute of Medical Science, University of Tokyo
Joint Usage/Research Center Program of the Institute for Frontier Life and Medical Sciences Kyoto University
Daiichi Sankyo Foundation of Life Science
Takeda Science Foundation
Joint Usage/Research Center on Tropical Disease
Institute of Tropical Medicine and Nagasaki University
Publisher
Oxford University Press (OUP)
Subject
Radiology, Nuclear Medicine and imaging,Instrumentation,Structural Biology
Cited by
3 articles.
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