Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli-Reference-Cited by-同舟云学术

Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli

Published:2021-04-07 Issue:6 Volume:85 Page:1464-1467
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Makino Yuriko¹²,Sato Hiroshi³,Noguchi Atsushi²,Ide Teruhiko²,Yatsunami Rie¹,Nakamura Satoshi¹⁴

Affiliation:

1. School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan

2. Life Science Research Laboratory, Tosoh Corp., Ayase, Kanagawa, Japan

3. Bioscience Division, Tosoh Corp., Minato-ku, Tokyo, Japan

4. National Institute of Technology, Numazu College, Numazu, Shizuoka, Japan

Abstract

ABSTRACT The α subunit of avian myeloblastosis virus reverse transcriptase (AMV-RT) is generated from the β-subunit by proteolysis, and the αβ heterodimer represents the active form. The codon-optimized gene was expressed in Escherichia coli, and an active αβ heterodimer was generated. The RNA amplification activity of the purified recombinant AMV-RT αβ heterodimer was similar to that of the native one.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbab057/37798584/zbab057.pdf

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