Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation-Reference-Cited by-同舟云学术

Analysis of the molecular evolution of histone variant H2A.Z using a linker-mediated complex strategy and yeast genetic complementation

Published:2021-10-29 Issue:1 Volume:86 Page:104-108
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Kitagawa Saho¹,Kusakabe Masayuki¹,Takahashi Daisuke¹,Narimiya Takumi¹,Nakabayashi Yu²,Seki Masayuki²,Horigome Chihiro¹^ORCID,Harata Masahiko¹

Affiliation:

1. Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai, Miyagi, Japan

2. Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University, Aoba-ku, Sendai, Miyagi, Japan

Abstract

ABSTRACT The histone variant H2A.Z is deposited into chromatin by specific machinery and is required for genome functions. Using a linker-mediated complex strategy combined with yeast genetic complementation, we demonstrate evolutionary conservation of H2A.Z together with its chromatin incorporation and functions. This approach is applicable to the evolutionary analyses of proteins that form complexes with interactors.

Funder

Japan Society for the Promotion of Science

Tohoku University

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbab190/41592214/zbab190.pdf

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