Mechanisms underlying enhanced IgA production in Peyer's patch cells by membrane vesicles derived from Lactobacillus sakei-Reference-Cited by-同舟云学术

Mechanisms underlying enhanced IgA production in Peyer's patch cells by membrane vesicles derived from Lactobacillus sakei

Published:2021-04-22 Issue:6 Volume:85 Page:1536-1545
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
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Author:

Miyoshi Yuki¹²,Saika Azusa²³,Nagatake Takahiro²,Matsunaga Ayu²⁴,Kunisawa Jun²³,Katakura Yoshio⁵,Yamasaki-Yashiki Shino²⁵^ORCID

Affiliation:

1. Chemistry, Materials and Bioengineering Major, Graduate School of Science and Engineering, Kansai University, Suita, Osaka, Japan

2. Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan

3. Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan

4. Department of Food and Life Science, School of Life and Environmental Science, Azabu University, Sagamihara, Kanagawa, Japan

5. Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka, Japan

Abstract

ABSTRACT We analyzed the mechanisms underlying enhanced IgA production in the cells of Peyer's patch cells via membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC 15893. Depletion of CD11c+ cells from Peyer's patch cells suppressed the enhanced IgA production mediated by membrane vesicles. Meanwhile, the stimulation of bone-marrow–derived dendritic cells with membrane vesicles increased gene expression of inducible nitric oxide synthase, retinaldehyde dehydrogenase 2, and several inflammatory cytokines. The production of nitric oxide and interleukin (IL)-6 by membrane vesicle stimulation was induced via Toll-like receptor 2 on bone marrow–derived dendritic cells. Inhibition of inducible nitric oxide synthase and retinaldehyde dehydrogenase 2, as well as the neutralization of IL-6 in Peyer's patch cells, suppressed the enhanced IgA production by membrane vesicle stimulation. Hence, nitric oxide, retinoic acid, and IL-6 induced by membrane vesicles play crucial roles in the enhanced IgA production elicited by membrane vesicles in Peyer's patch cells.

Funder

Japan Society for the Promotion of Science

Research Group Fund of Kansai University

Japan Agency for Medical Research and Development

The Ministry of Health, Labour, and Welfare of Japan

Public/Private R&D Investment Strategic Expansion Program

Terumo Foundation for Life Sciences and Arts

Ono Medical Research Foundation

Canon Foundation

Publisher

Oxford University Press (OUP)

Subject