Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3-galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor-Reference-Cited by-同舟云学术

Chemo-enzymatic synthesis of Lacto-N-biose I catalyzed by β-1,3-galactosidase from Bacillus circulans using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside as a glycosyl donor

Published:2021-04-20 Issue:7 Volume:85 Page:1716-1719
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Ohnuma Takayuki¹²,Taku Tomoki¹,Nagatani Takeshi¹,Horii Atsushi¹,Imaoka Shun¹,Tanaka Tomonari³

Affiliation:

1. Department of Advanced Bioscience, Kindai University, Nara, Japan

2. Agricultural Technology and Innovation Research Institute (ATIRI), Kindai University, Nara, Japan

3. Department of Biobased Materials Science, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, Japan

Abstract

ABSTRACT Chemo-enzymatic synthesis of lacto-N-biose I (LNB) catalyzed by β-1,3-galactosidase from Bacillus circulans (BgaC) has been developed using 4,6-dimethoxy-1,3,5-triazin-2-yl β-galactopyranoside (DMT-β-Gal) and GlcNAc as the donor and acceptor substrates, respectively. BgaC transferred the Gal moiety to the acceptor, giving rise to LNB. The maximum yield of LNB was obtained at the acceptor : donor substrate ratio of 1:30.

Funder

Agricultural Technology and Innovation Research Institute

Kindai University

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbab071/37904482/zbab071.pdf

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