Enzymatic determination of d-alanine with l-alanine dehydrogenase and alanine racemase-Reference-Cited by-同舟云学术

Enzymatic determination of d-alanine with l-alanine dehydrogenase and alanine racemase

Published:2021-08-24 Issue:11 Volume:85 Page:2221-2223
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Ashida Hiroyuki¹,Sawa Yoshihiro²,Yoshimura Tohru³

Affiliation:

1. Interdisciplinary Center for Science Research, Shimane University, Japan

2. Faculty of Life and Environmental Science, Shimane University, Japan

3. Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Japan

Abstract

ABSTRACT An enzymatic assay system of d-Ala, which is reported to affect the taste, was constructed using alanine racemase and l-alanine dehydrogenase. d-Ala is converted to l-Ala by alanine racemase and then deaminated by l-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. Using the assay system, the d-Ala contents of 7 crustaceans were determined.

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbab148/40354842/zbab148.pdf

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