Analysis of the light regulatory mechanism in carotenoid production in Rhodosporidium toruloides NBRC 10032-Reference-Cited by-同舟云学术

Analysis of the light regulatory mechanism in carotenoid production in Rhodosporidium toruloides NBRC 10032

Published:2021-06-14 Issue:8 Volume:85 Page:1899-1909
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Pham Khanh Dung¹,Hakozaki Yuuki¹,Takamizawa Takeru¹,Yamazaki Atsushi²,Yamazaki Harutake³,Mori Kazuki⁴,Aburatani Sachiyo⁵,Tashiro Kosuke⁶,Kuhara Satoru⁷,Takaku Hiroaki³,Shida Yosuke¹,Ogasawara Wataru¹

Affiliation:

1. Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan

2. Biological Resource Center, National Institute of Technology and Evaluation (NITE), Chiba, Japan

3. Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan

4. Cell Innovator Co., Ltd., Fukuoka, Japan

5. AIST-Waseda University Computational Bio Big-Data Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo, Japan

6. Faculty of Agriculture, Kyushu University, Fukuoka, Japan

7. Graduate School of Genetic Resource Technology, Kyushu University, Fukuoka, Japan

Abstract

ABSTRACT Light stimulates carotenoid production in an oleaginous yeast Rhodosporidium toruloides NBRC 10032 by promoting carotenoid biosynthesis genes. These genes undergo two-step transcriptional activation. The potential light regulator, Cryptochrome DASH (CRY1), has been suggested to contribute to this mechanism. In this study, based on KU70 (a component of nonhomologous end joining (NHEJ)) disrupting background, CRY1 disruptant was constructed to clarify CRY1 function. From analysis of CRY1 disruptant, it was suggested that CRY1 has the activation role of the carotenogenic gene expression. To obtain further insights into the light response, mutants varying carotenoid production were generated. Through analysis of mutants, the existence of the control two-step gene activation was proposed. In addition, our data analysis showed the strong possibility that R. toruloides NBRC 10032 is a homo-diploid strain.

Funder

New Energy and Industrial Technology Development Organization

Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

http://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbab109/38983640/zbab109.pdf

Reference51 articles.

1. The CRY1 blue light photoreceptor of Arabidopsis interacts with phytochrome a in vitro;Ahmad;Mol Cell,1998

2. Influence of carbon and nitrogen sources on Flavobacterium growth and zeaxanthin biosynthesis;Alcantara;J Ind Microbiol Biotechnol,1999