Litchi chinensis inspired nanoformulations: a synergy guided approach for unraveling promising cytotoxic attributes of metal and nonmetal conjugates

Author:

Hussain Amina12,Safdar Naila1ORCID,Ain Noor-ul1,Abbasi Rashda3,Yasmin Azra1

Affiliation:

1. Microbiology and Biotechnology Research Laboratory, Department of Biotechnology, Fatima Jinnah Women University, Pakistan Old Presidency, The Mall, Rawalpindi 46000, Pakistan

2. Department of Environmental Sciences, Fatima Jinnah Women University, Pakistan Old Presidency, The Mall, Rawalpindi 46000, Pakistan

3. Cancer Biology Institute of Biomedical and Genetic Engineering (IBGE), G-9/1, Islamabad 44000, Pakistan

Abstract

Abstract In present study, diverse Litchi chinensis-mediated nanostructures in combination with 5-fluorouracil drug were fabricated viz. Au, Se, Ag, Ag–Se, Ag–Au, 5-FU Ag–Se and 5-FU Ag–Au with subsequent characterization and scrutinization of their anticarcinogenic capabilities. UV–Visible spectroscopic analysis confirmed the state transition for each precursor salt. XRD and transmission electron microscopy analysis revealed spherical/quasispherical nanostructures with monoclinic crystalline organization ranged between 18 nm and 38 nm. FTIR analysis revealed fabricated nanoparticles to be capped with various phytoconstituents. DLS and Zeta potential analysis of unloaded and drug-loaded bielemental nanoparticles (BNPs) showed comparatively large hydrodynamic particle size distribution and sufficient stability of nanoparticles. BNPs showed promising lethality concentrations for brine shrimp (LC50 < 2 μg/ml) and antitumor (LC50 < 10 μg/ml) assessments. These findings were in positive correlation with the antioxidant inhibitory concentrations IC50 (74.2–180.1 μg/ml) of the tested entities. Ag–Se and Ag–Au were loaded with 5-FU (loading efficiency of 47% ± 1.14 and 25% ± 0.32, respectively) in light of their promising cytotoxic actions. All nanostructures showed profound hemocompatibility with maximum hemolytic activity as low as 2.4%. Highly significant difference (P < 0.01) was observed in antineoplastic potentials of unloaded and 5-FU loaded BNPs against HepG2 and HT144, with most substantial IC50 for 5-FU Ag–Au (8.95 ± 2.86 μg/ml). 5-FU Ag–Au was identified as a significant inducer of DNA fragmentation with maximum relative tail moment (HepG2: 3.45 ± 0.21) among all treatments.

Funder

National Research Program for Universities

Publisher

Oxford University Press (OUP)

Subject

Health, Toxicology and Mutagenesis,Toxicology

Reference57 articles.

1. Cytotoxicity of Ag, Au and Ag-Au bimetallic nanoparticles prepared using golden rod (Solidago canadensis) plant extract;Botha;Sci Rep,2019

2. Photo-mediated optimized synthesis of silver nanoparticles using the extracts of outer shell fibre of Cocos nucifera L. fruit and detection of its antioxidant, cytotoxicity and antibacterial potential, Saudi;Das;J Biol Sci,2020

3. Anticancer, antimicrobial, antioxidant, and catalytic activities of green-synthesized silver and gold nanoparticles using Bauhinia purpurea leaf extract;Vijayan;Bioprocess Biosyst Eng,2019

4. Green synthesis and characterizations of gold nanoparticles using Thyme and survey cytotoxic effect, antibacterial and antioxidant potential;Hamelian;J Photochem Photobiol B Biol,2018

5. Non-cytotoxic effect of green synthesized silver nanoparticles and its antibacterial activity;Senthil;J Photochem Photobiol B Biol,2017

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3