Rapid Screening and Characterization of Triterpene Saponins from the Root of Phytolacca acinosa Roxb by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

Author:

Ling Yun1ORCID,Zhu Yue1,Gan Qiao1,Li Guilan1,Luo Zefan1,Pan Yali1,Zhao Lian1,Lei Zhineng1,He Xin1,Zeng Ming1,Liu Wenbo1

Affiliation:

1. School of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People’s Republic of China

Abstract

Abstract Triterpene saponins (TSs) are important bioactive constituents with structural diversity widely distributed in many plants. The root of Phytolacca acinosa Roxb (RPa) has been used as a traditional Chinese medicine. However, TSs as the main active ingredients in RPa have not been fully characterized. Here, we profiled TSs from RPa by high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS). We tentatively identified 29 TSs, including 13 that had not been reported previously from this plant. This study indicates that HPLC-ESI-QTOF-MS/MS is an effective and rapid method for the characterization of complicated TSs in herbal extracts.

Funder

Natural Science Foundation of Jiangxi Province of China

Educational Department of Jiangxi Province of China

Health Commission of Jiangxi Province

College Students’ Innovative Entrepreneurial Training Plan Program

Publisher

Oxford University Press (OUP)

Subject

General Medicine,Analytical Chemistry

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