Affiliation:
1. Hangzhou Institute for Food and Drug Control, Hangzhou 310017, P.R. China
2. Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Farm Produces, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, P.R. China
Abstract
Abstract
This study established a rapid and reliable method to determine chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) residues in Chinese gelatin medicines. CAP, TAP and FF were extracted from medicine samples using 2% (v/v) ammonium hydroxide in acetonitrile. Trypsin was used to eliminate the matrix effect caused by protein components in gelatin medicines, whereas anhydrous sodium sulfate, C18-N and NH2-PSA adsorbents were applied to reduce matrix effect induced by other components. The analytical method of these drugs was optimized on ultra high-performance liquid chromatography-mass spectrometer (UHPLC-MS/MS) through the analysis of their standard linearity and regression. The optimized extraction and analytical method were validated in one Chinese gelatin medicine sample (Colla corii asini, E Jiao) with three fortification levels (2, 5 and 10 μg/kg), and the recoveries of these drug residues ranged of 87.6–102.7%. The limit of detection and quantification of CAP, TAP and FF in the sample were 0.2 and 0.5 μg/kg, 0.4 and 1.5 μg/kg, and 0.5 and 1.5 μg/kg, respectively. A total of 30 Chinese gelatin medicine samples were analyzed using the established method. No drug residues were found in these samples except for one Testudinis Carapacis et Plastri (1.67 μg/kg FF) and one turtle shell glue (2.55 μg/kg FF).
Funder
Major Agricultural Projects of Zhejiang Province
Social Development Program of Hangzhou Science and Technology Committee
the program for the Science and Technology plan of Zhejiang Food and Drug Administration
Zhejiang Provincial Natural Science Foundation of China
Open Foundation of Beijing Advanced Innovation Center for Food Nutrition and Human Health
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
General Medicine,Analytical Chemistry