Traditional Horticulture Practices Increase the Production of Selected Withanolides in Withania Somnifera (L.) Dunal—A RP-UFLC Analysis

Author:

Ankad Gireesh M1,Pai Sandeep R2,Hiremath Jagadishchandra3,Hegde Harsha V1

Affiliation:

1. ICMR—National Institute of Traditional Medicine, Nehru Nagar, Belgaum 590010, India

2. Department of Botany, Rayat Shikshan Sansthas, Dada Patil Mahavidyalaya, Karjat 414402, India

3. Department of Spices, Medicinal and Aromatic plants, Kittur Rani Chennamma of Horticulture, Arabhavi 591218, India

Abstract

Abstract The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.

Publisher

Oxford University Press (OUP)

Subject

General Medicine,Analytical Chemistry

Reference22 articles.

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