Evaluation of anticoagulants and hemocyte-maintaining solutions for the study of hemolymph components in the spiny lobster Panulirus argus (Latreille, 1804) (Decapoda: Achelata: Palinuridae)

Author:

Perdomo-Morales Rolando1ORCID,Montero-Alejo Vivian1,Rodríguez-Viera Leandro2,Perera Erick3

Affiliation:

1. Centro de Investigación y Desarrollo de Medicamentos, CP, La Habana, Cuba

2. Centro de Investigaciones Marinas, Universidad de La Habana, La Habana, Cuba

3. Grupo de Nutrigenómica y Endocrinología del Crecimiento de Peces, Instituto de Acuicultura Torre de la Sal, Ribera de Cavanes, S/N, Consejo Superior de Investigaciones Científicas, Castellón, Spain

Abstract

AbstractFunctional studies on humoral or cellular responses in the hemolymph of crustaceans require the selection of suitable anticoagulant- and hemocyte-maintaining solutions. We studied the suitability of several anticoagulant- and hemocyte-maintaining solutions in the spiny lobster Panulirus argus (Latreille, 1804), with emphasis in the preservation of hemocyte number and viability. It was found that the modified Alsever solution was the ideal anticoagulant, while modified L-15 medium and Panulirus argus saline (PAS) were the best hemocyte-maintaining solutions. It is striking that whereas avoiding plasma clotting is relatively simple to achieve, avoiding lysis and aggregation of hemocytes could be challenging and variable among closely related crustaceans. The reasons are hardly known and might indicate different composition or sensitivity of both membrane-bound and soluble mediators in any of the three types of hemocytes identified among decapod crustaceans. Hemolymph volume average in P. argus was 10.5% of fresh body weight (more than 50 ml per adult individual), which makes this species an attractive model for functional studies of hemolymph components in crustaceans.

Publisher

Oxford University Press (OUP)

Subject

Aquatic Science

Reference33 articles.

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2. N-ethylmaleimide inhibition of horseshoe crab hemocyte agglutination;Bryan;Science,1964

3. Crecimiento y mortalidad de la langosta espinosa (Panulirus argus) en la plataforma suroccidental de Cuba;Cruz;Revista Cubana de Investigaciones Pesqueras,1981

4. Haemolymph protein composition and copper levels in decapod crustaceans;Depledge;Helgoländer Meeresuntersuchungen,1989

5. Action of various anticoagulants on hemolymphs of lobsters and spiny lobsters;Durliat;Biological Bulletin,1981

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