Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)-Reference-Cited by-同舟云学术

Culturing embryonic cells from the parthenogenetic clonal marble crayfish (Marmorkrebs) Procambarus virginalis Lyko, 2017 (Decapoda: Astacidea: Cambaridae)

Published:2019-09-26 Issue:6 Volume:39 Page:758-763
ISSN:0278-0372
Container-title:Journal of Crustacean Biology
language:en
Short-container-title:

Author:

Deleon Heriberto¹,Garcia Juan¹,Silva Dionn Carlo¹,Quintanilla Oscar¹,Faulkes Zen¹,Thomas John M¹²³

Affiliation:

1. Department of Biology, The University of Texas Rio Grande Valley, Edinburg, TX, USA

2. Center for Vector Borne Disease, The University of Texas Rio Grande Valley, Edinburg, TX, USA

3. School of Medicine, The University of Texas Rio Grande Valley, Edinburg, TX, USA

Abstract

Abstract The parthenogenetic marbled crayfish, or Marmorkrebs (Procambarus virginalis Lyko 2017), is an emerging model organism. We describe a method to isolate cells from early-stage embryos and culture them in vitro. The identity of the cells was confirmed by sequencing the cytochrome c oxidase subunit I (COI) gene. This technique can be applied for use in the manipulation of embryonic parthenogenetic crayfish cells.

Publisher

Oxford University Press (OUP)

Subject

Aquatic Science

Link

http://academic.oup.com/jcb/article-pdf/39/6/758/31112728/ruz063.pdf

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