Evidence for vocal diversity during physical interference at the perch in sympatric Carollia species (Chiroptera: Phyllostomidae): a key to social organization and species coexistence?

Abstract

Abstract Vocal diversity might reflect the social organization and sustain the coexistence of cryptic species in sympatry. To test the extent to which vocal behaviour separates sympatric, cryptic species of a bat radiation, we compared vocalizations of genetically confirmed Carollia castanea, Carollia sowelli and Carollia perspicillata emitted by intraspecific dyads of identified individuals during corresponding physical interference interactions at the perch. Video analysis revealed a similar behaviour and interaction time across species. A sonagram-based visual classification of vocalization syllables of uninterrupted frequency–time contour discriminated 21 syllable classes. Class usage and distribution of the four shared classes differed across species. Carollia sowelli emitted the lowest number of classes in total and per interaction across species and displayed a limited number of syllable compositions in bouts. Discriminant analyses of syllables of a common, shared class provided evidence for species distinctiveness and individual-specific signatures. In general, sex did not account for data variability. The present vocalizations combine syllables reported from aggressive and submissive contexts in C. perspicillata and might express experienced ambivalence during interference at the perch. The diversity of vocal behaviour across congeners is discussed as arising from different ecological pressures during allopatric speciation and as an indicator of differences in species social organization.