Mycobiome of the external ear canal of healthy cows

Author:

Díaz Leyna1ORCID,Castellá Gemma1ORCID,Bragulat M Rosa1ORCID,Paytuví-Gallart Andreu2ORCID,Sanseverino Walter2ORCID,Cabañes F Javier1ORCID

Affiliation:

1. Veterinary Mycology Group, Department of Animal Health and Anatomy, Universitat Autònoma de Barcelona , Bellaterra, Catalonia , Spain

2. Sequentia Biotech SL , Barcelona, Catalonia , Spain

Abstract

Abstract Malassezia yeasts belong to the normal skin microbiota of a wide range of warm-blooded animals. However, their significance in cattle is still poorly understood. In the present study, the mycobiota of the external ear canal of 20 healthy dairy Holstein cows was assessed by cytology, culture, PCR, and next-generation sequencing. The presence of Malassezia was detected in 15 cows by cytology and PCR. The metagenomic analysis revealed that Ascomycota was the predominant phylum but M. pachydermatis the main species. The Malassezia phylotype 131 was detected in low abundance. Nor M. nana nor M. equina were detected in the samples.

Funder

UAB

Publisher

Oxford University Press (OUP)

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