Optimized knock-in of point mutations in zebrafish using CRISPR/Cas9

Author:

Prykhozhij Sergey V1,Fuller Charlotte2,Steele Shelby L3,Veinotte Chansey J1,Razaghi Babak1,Robitaille Johane M1,McMaster Christopher R1,Shlien Adam4,Malkin David4,Berman Jason N1

Affiliation:

1. Departments of Pediatrics, Microbiology & Immunology, and Pathology, Dalhousie University, Halifax, NS, B3H 4R2, Canada

2. Michael G. DeGroote School of Medicine, McMaster University,Hamilton, ON, L8S4L8, Canada

3. Appili Therapeutics, Halifax, NS, B3H 4R2, Canada

4. Departments of Pediatrics and Medical Biophysics, University of Toronto, Toronto, ON, M5G 1X8, Canada

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference34 articles.

1. Zebrafish genome engineering using the CRISPR–Cas9 System;Li;Trends Genet.,2016

2. Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9;Ceasar;Biochim. Biophys. Acta,2016

3. Understanding and editing the zebrafish genome;Varshney;Methods Mol. Biol.,2015

4. A guide to computational tools and design strategies for genome editing experiments in zebrafish using CRISPR/Cas9;Prykhozhij;Zebrafish,2015

5. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs;Gagnon;PLoS One,2014

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