Liquid biopsy in gliomas: A RANO review and proposals for clinical applications

Author:

Soffietti Riccardo1ORCID,Bettegowda Chetan2,Mellinghoff Ingo K3,Warren Katherine E4,Ahluwalia Manmeet S5,De Groot John F6,Galanis Evanthia7ORCID,Gilbert Mark R8,Jaeckle Kurt A9,Le Rhun Emilie10,Rudà Roberta11,Seoane Joan12,Thon Niklas13,Umemura Yoshie14,Weller Michael15ORCID,van den Bent Martin J16,Vogelbaum Michael A17,Chang Susan M18,Wen Patrick Y19

Affiliation:

1. Division of Neuro-Oncology, Department of Neuroscience, University and City of Health and Science Hospital , Turin , Italy

2. Department of Neurosurgery, Johns Hopkins University School of Medicine , Baltimore , Maryland, USA

3. Department of Neurology, Memorial Sloan Kettering Cancer Center New York , USA

4. Department of Pediatric Oncology, Dana-Farber/Boston Children’s Cancer and Blood Disorders Center, Dana-Farber Cancer Institute , Boston, Massachusetts , USA

5. Brain Tumor and Neuro-Oncology Center, Cleveland Clinic , Cleveland, Ohio , USA

6. Department of Neuro-Oncology, University of Texas, MD Anderson Cancer Center Houston , Houston, Texas , USA

7. Department of Radiation Oncology , Mayo Clinic, Rochester, Minnesota , USA

8. Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health , Bethesda, Maryland , USA

9. Department of Neurology, Mayo Clinic Florida , Jacksonville, Florida , USA

10. Departments of Neurology & Neurosurgery, Clinical Neuroscience Center, University Hospital and University of Zurich , Zurich , Switzerland

11. Department of Neurology, Castelfranco Veneto/Treviso Hospital and Division of Neuro-Oncology, Department of Neuroscience, University of Turin, Turin , Italy

12. Vall d’Hebron Institute of Oncology (VHIO) University Hospital, Universitat Autònoma de Barcelona, ICREA,CIBERONC , Barcelona , Spain

13. Division of Neuro-Oncology, Department of Neurosurgery, Ludwig Maximilians University School of Medicine , Munich , Germany

14. Division of Neuro-Oncology, Department of Neurology, University of Michigan , Ann Arbor, Michigan , USA

15. Department of Neurology, Clinical Neuroscience Center, University Hospital and University of Zurich , Zurich , Switzerland

16. Department of Neurology, Brain Tumor Center at Erasmus MC Cancer Institute, University Medical Center Rotterdam , Rotterdam , The Netherlands

17. Department of Neurosurgery, Cleveland Clinic , Cleveland , Ohio, USA

18. Division of Neuro-Oncology, University of California San Francisco , San Francisco, California , USA

19. Center for Neuro-Oncology, Dana-Farber/Brigham and Women’s Cancer Center, Harvard Medical School , Boston, Massachusetts , USA

Abstract

AbstractBackgroundThere is an extensive literature highlighting the utility of blood-based liquid biopsies in several extracranial tumors for diagnosis and monitoring.MethodsThe RANO (Response Assessment in Neuro-Oncology) group developed a multidisciplinary international Task Force to review the English literature on liquid biopsy in gliomas focusing on the most frequently used techniques, that is circulating tumor DNA, circulating tumor cells, and extracellular vesicles in blood and CSF.ResultsctDNA has a higher sensitivity and capacity to represent the spatial and temporal heterogeneity in comparison to circulating tumor cells. Exosomes have the advantages to cross an intact blood-brain barrier and carry also RNA, miRNA, and proteins. Several clinical applications of liquid biopsies are suggested: to establish a diagnosis when tissue is not available, monitor the residual disease after surgery, distinguish progression from pseudoprogression, and predict the outcome.ConclusionsThere is a need for standardization of biofluid collection, choice of an analyte, and detection strategies along with rigorous testing in future clinical trials to validate findings and enable entry into clinical practice.

Publisher

Oxford University Press (OUP)

Subject

Cancer Research,Neurology (clinical),Oncology

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