Influence of Storage Conditions on SARS-CoV-2 Nucleic Acid Detection in Throat Swabs-Reference-Cited by-同舟云学术

Influence of Storage Conditions on SARS-CoV-2 Nucleic Acid Detection in Throat Swabs

Published:2020-05-19 Issue:2 Volume:222 Page:203-205
ISSN:0022-1899
Container-title:The Journal of Infectious Diseases
language:en
Short-container-title:

Author:

Li Lin¹²,Li Xiao¹²,Guo Zhendong¹²,Wang Zhongyi¹²,Zhang Ke¹²,Li Chao³,Wang Changjun²⁴,Zhang Shoufeng¹²

Affiliation:

1. Academy of Military Medical Sciences, Academy of Military Sciences, Beijing, China

2. Wuhan Huoshenshan Hospital, Wuhan, China

3. Institute of Systems Engineering, Academy of Military Sciences, Tianjin, China

4. Centers for Disease Control and Prevention of the People's Liberation Army, Beijing, China

Abstract

Abstract The detection of SARS-CoV-2 infection is the premise of quarantine. In many countries or areas, samples need to be shipped or inactivated before SARS-CoV-2 testing. In this study, we checked the influence of sample storage conditions on SARS-CoV-2 nucleic acid testing results, including sample inactivation time, storage temperature, and storage time. All of these conditions caused an increase in the cycle threshold values of the nucleic acid tests and led to the misclassification of at least 10.2% of positive cases as negative or suspected. The results highlight the importance of immediate testing of samples for SARS-CoV-2 nucleic acid detection.

Funder

National Key Research and Development Program

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

Link

http://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiaa272/33317960/jiaa272.pdf

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