New Window Into Hepatitis B in Africa: Liver Sampling Combined With Single-Cell Omics Enables Deep and Longitudinal Assessment of Intrahepatic Immunity in Zambia-Reference-Cited by-同舟云学术

New Window Into Hepatitis B in Africa: Liver Sampling Combined With Single-Cell Omics Enables Deep and Longitudinal Assessment of Intrahepatic Immunity in Zambia

Published:2024-02-09 Issue: Volume: Page:
ISSN:0022-1899
Container-title:The Journal of Infectious Diseases
language:en
Short-container-title:

Author:

Musonda Taonga¹^ORCID,Wallace Michael S²,Patel Hailey²,Martin Owen P²,Oetheimer Christopher²,Mwakamui Simutanyi¹,Sinkala Edford¹³,Nsokolo Bright⁴,Kanunga Annie¹⁵,Lauer Georg²,Chung Raymond T²,Wandeler Gilles⁶⁷,Bhattacharya Debika⁸^ORCID,Kelly Paul¹⁹^ORCID,Alatrakchi Nadia²,Vinikoor Michael J³⁵¹⁰

Affiliation:

1. Tropical Gastroenterology and Nutrition Group, University of Zambia , Lusaka , Zambia

2. Division of Gastroenterology, Massachusetts General Hospital , Boston, Massachusetts , USA

3. Department of Medicine, University Teaching Hospital , Lusaka , Zambia

4. Department of Medicine, Levy Mwanawasa University Teaching Hospital , Lusaka , Zambia

5. Department of Research, Centre for Infectious Disease Research in Zambia , Lusaka , Zambia

6. Department of Infectious Diseases, Bern University Hospital, University of Bern , Bern , Switzerland

7. Institute of Social and Preventive Medicine, University of Bern , Bern , Switzerland

8. Division of Infectious Diseases, University of California Los Angeles , Los Angeles, California , USA

9. Blizard Institute, Queen Mary University of London , London , United Kingdom

10. Division of Infectious Diseases, University of Alabama Birmingham , Birmingham, Alabama , USA

Abstract

Abstract In Lusaka, Zambia, we introduced liver fine-needle aspiration biopsy (FNAB) into a research cohort of adults with treatment-naive chronic hepatitis B virus (HBV) infection, with and without human immunodeficiency virus (HIV) coinfection, as well as with acute HBV infection. From 117 enrollment and 47 longitudinal FNABs (at 1-year follow-up), we established participant acceptability and safety. We also demonstrated the quality of the material through single-cell RNA sequencing of selected enrollment FNAs, which revealed a range of immune cells. This approach can drive new insights into HBV immunology, informing cure strategies, and can improve our understanding of HBV natural history in Africa.

Funder

National Institutes of Health

Swiss National Science Foundation

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiae054/56844615/jiae054.pdf

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