Human Papillomavirus 16 Lineage A Variants Associated With Persistent Genital Infections in Men: The HPV Infection in Men (HIM) Study

Author:

Ferreira Matthew Thomas1,Mendoza López Rossana Veronica12,Gonçalves Milena Giulia12,Ferreira Silvaneide1,Sirak Bradley3,Baggio Maria Luizai1,Lazcano-Ponce Eduardo4,Nyitray Alan G56,Giuliano Anna R3,Villa Luisa L127,Sichero Laura12,Lin Huiyi,Messina Jane,Campbell Christine Pierce,Sirak Bradley,Gage Christine,Ingles Donna J,Isaacs Kim,Kennedy Kayoko,Bobanic Andrea,Rahman Shams,Schabath Matthew,Nyitray Alan,Rathwell Julie,de Paula Lenice Galan,Cintra Ricardo,Cernicchiaro Filomena,Ribeiro Graça,Otero Rosária,Bocalon Roberta,Antunes Juliana,Silva Fernanda,Terreri Rossana,Valdez Aurelio Cruz,de Jesús Alvear Vásquez René,Juárez Oscar Rojas,del Carmen González Sosa Rossana,Vences Rosangel Ríos,Segura Martha Huerta,Galván Alicia Rodríguez,Rodríguez Paula Román,Vélez Ana Laura Landa,García Griselda Díaz,Abarca Verónica Chávez,Quevedo Gisela Flores,del Pilar Hernández Nevárez María,Martínez Guillermina Sánchez,Rojas Adriana Ortiz,Flores Carlos Omar Barrera,Mangone Flavia Rotea,Pavanelli Ana Carolina,

Affiliation:

1. Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas da Faculdade de Medicina da Universidade de São Paulo , Sao Paulo , Brazil

2. Comprehensive Center for Precision Oncology, Universidade de Sao Paulo , São Paulo , Brazil

3. Center for Immunization and Infection Research in Cancer, and Department of Cancer Epidemiology, H. Lee Moffitt Cancer Center and Research Institute , Tampa, Florida , USA

4. Center for Population Health Research, Instituto Nacional de Salud Pública, Instituto Mexicano del Seguro Social , Cuernavaca , Mexico

5. Health Intervention Sciences Group/Center for AIDS Intervention Research, Medical College of Wisconsin , Milwaukee, Wisconsin , USA

6. Clinical Cancer Center, Medical College of Wisconsin , Milwaukee, Wisconsin , USA

7. Department of Radiology and Oncology, Faculdade de Medicina da Universidade de Sao Paulo , Sao Paulo , Brazil

Abstract

Abstract Background Human papillomavirus (HPV) 16 non-A lineage variants have higher carcinogenic potential for cervical cancer. HPV-16 variants natural history among males is not established. We evaluated HPV-16 variants prevalence and persistence in the external genitalia of men enrolled in the prospective HPV Infection in Men (HIM) Study. Methods The HIM Study included men from the United States, Brazil, and Mexico. HPV-16 variants were distinguished using polymerase chain reaction sequencing. The prevalence of HPV-16 variants was assessed, and associations with infection persistence were estimated. Results We characterized the HPV-16 variants for 1700 genital swab samples from 753 men and 22 external genital lesions in 17 men. The prevalence of HPV-16 lineages differed by country and marital status (P < .001). Overall, 90.9% of participants harbored lineage A variants. The prevalence of non-A lineages was heterogenous among countries. HPV-16 lineage A variants were associated with a 2.69-fold increased risk of long-term persistent infections compared with non-A lineages. All high-grade penile intraepithelial neoplasia harbored lineage A variants and occurred in the context of long-term persistent infections with the same variants. Conclusions The prevalence and persistence of HPV-16 variants observed at the male external genitalia suggest differences in the natural history of these variants between men and women, which may be associated with intrinsic differences in the infected genital epithelia.

Funder

São Paulo Research Foundation

Conselho Nacional de Desenvolvimento Científico e Tecnológico

National Cancer Institute

National Institutes of Health

Ludwig Institute for Cancer Research

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

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