Genetic Profiling of Plasmodium ovale wallikeri Relapses With Microsatellite Markers and Whole-Genome Sequencing
Author:
Joste Valentin12ORCID, Colard-Itté Emma2, Guillochon Émilie1, Ariey Frédéric3, Coppée Romain4ORCID, Clain Jérôme12, Houzé Sandrine12, Aboubacar Ahmed, Angebault Cécile, Angoulvant Adela, Argy Nicolas, Azjenberg Daniel, Belkadi Ghania, Bellanger Anne-Pauline, Bemba Dieudonné, Blaize Marion, Botterel Françoise, Bougnoux Marie-Elisabeth, Brun Sophie, Buret Bernadette, Chevrier Sylviane, Clauser Sylvain, Dahane Naima, Dannaoui Eric, Dard Céline, Dardé Marie-Laure, de Gentile Ludovic, de Suremain Nathalie, Debourgogne Anne, Delaval Anne, Deleplancque Anne-Sophie, Desoubeaux Guillaume, Durand Rémy, Durieux Marie-Fleur, Dutoit Emmanuel, Eloy Odile, Fenneteau Odile, Gargala Gilles, Godineau Nadine, Guennouni Nadia, Guinard Jérôme, Hamane Samia, Herault Etienne, Larreché Sébastien, Lavergne Rose-Anne, Marteau Anthony, Mazars Edith, Moreno-Sabater Alicia, Morio Florent, Nourrisson Céline, Perraud-Cateau Estelle, Pons Denis, Pull Lauren, Quinio Dorothée, Raffenot Didier, Silva Muriel, Thellier Marc, Tielli Alexandra, Toubas Dominique,
Affiliation:
1. Mère et enfant en milieu tropical, Institut Recherche pour le Développement, Université Paris Cité 2. Centre National de Référence du Paludisme, Assistance Publique–Hôpitaux de Paris, Hôpital Bichat–Claude-Bernard 3. Inserm U1016, Institut Cochin, Laboratoire de parasitologie-mycologie, Hôpital Cochin, Assistance Publique–Hôpitaux de Paris, Université de Paris Cité 4. Université Paris Cité and Sorbonne Paris Nord, Inserm, IAME , Paris , France
Abstract
Abstract
Like Plasmodium vivax, both Plasmodium ovale curtisi and Plasmodium ovale wallikeri have the ability to cause relapse in humans, defined as recurring asexual parasitemia originating from liver-dormant forms subsequent to a primary infection. Here, we investigated relapse patterns in P ovale wallikeri infections from a cohort of travelers who were exposed to the parasite in sub-Saharan Africa and then experienced relapses after their return to France. Using a novel set of 8 highly polymorphic microsatellite markers, we genotyped 15 P ovale wallikeri relapses. For most relapses, the paired primary and relapse infections were highly genetically related (with 12 being homologous), an observation that was confirmed by whole-genome sequencing for the 4 relapses we further studied. This is, to our knowledge, the first genetic evidence of relapses in P ovale spp.
Funder
French Ministry of Health
Publisher
Oxford University Press (OUP)
Subject
Infectious Diseases,Immunology and Allergy
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