Hemolytic Membrane Vesicles of Group B Streptococcus Promote Infection

Author:

Armistead Blair12ORCID,Quach Phoenicia2,Snyder Jessica M3,Santana-Ufret Verónica2,Furuta Anna12,Brokaw Alyssa12,Rajagopal Lakshmi124

Affiliation:

1. Department of Global Health, University of Washington, Seattle, Washington, USA

2. Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, Washington, USA

3. Department of Comparative Medicine, University of Washington School of Medicine, Seattle, Washington, USA

4. Department of Pediatrics, University of Washington School of Medicine, Seattle, Washington, USA

Abstract

Abstract Background Group B streptococci (GBS) are β-hemolytic, Gram-positive bacteria associated with fetal injury, preterm birth, spontaneous abortion, and neonatal infections. A key factor promoting GBS virulence is the β-hemolysin/cytolysin, a pigmented ornithine rhamnolipid (also known as granadaene) associated with the bacterial surface. Methods A previous study indicated that GBS produce small structures known as membrane vesicles (MVs), which contain virulence-associated proteins. In this study, we show that GBS MVs are pigmented and hemolytic, indicating that granadaene is functionally active in MVs. Results In addition, MVs from hyperhemolytic GBS induced greater cell death of neutrophils, T cells, and B cells compared with MVs from isogenic nonhemolytic GBS, implicating MVs as a potential mechanism for granadaene-mediated virulence. Finally, hemolytic MVs reduced oxidative killing of GBS and aggravated morbidity and mortality of neonatal mice infected with GBS. Conclusions These studies, taken together, reveal a novel mechanism by which GBS deploy a crucial virulence factor to promote bacterial dissemination and pathogenesis.

Funder

National Institutes of Health

Seattle Children's Research Institute

NIH Training Grants

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

Reference44 articles.

1. Estimates of the burden of group B streptococcal disease worldwide for pregnant women, stillbirths, and children;Seale;Clin Infect Dis,2017

2. Group B streptococcal disease in infants aged younger than 3 months: systematic review and meta-analysis;Edmond;Lancet,2012

3. A hemolytic pigment of group B streptococcus allows bacterial penetration of human placenta;Whidbey;J Exp Med,2013

4. Granadaene: proposed structure of the group B streptococcus polyenic pigment;Rosa-Fraile;Appl Environ Microbiol,2006

5. Group B streptococcus circumvents neutrophils and neutrophil extracellular traps during amniotic cavity invasion and preterm labor;Boldenow;Sci Immunol,2016

Cited by 23 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3