Rapid Antigen and Antibody Microfluidic Immunofluorescence Assays Compared to Culture, PCR, and Laboratory Reference Tests: Performance in a Longitudinal Cohort-Reference-Cited by-同舟云学术

Rapid Antigen and Antibody Microfluidic Immunofluorescence Assays Compared to Culture, PCR, and Laboratory Reference Tests: Performance in a Longitudinal Cohort

Published:2023-06-24 Issue:8 Volume:228 Page:1066-1070
ISSN:0022-1899
Container-title:The Journal of Infectious Diseases
language:en
Short-container-title:

Author:

Dalmat Ronit R¹²^ORCID,Hao Linhui³⁴,Prabhu Roshni¹,Rechkina Elena¹,Hamilton Daphne¹,Ikuma Matthew H¹,Bauer Marie¹,Gale Michael⁴⁵,Cantera Jason L⁶,Ball Alexey S⁶,Grant Benjamin D⁶,Drain Paul K¹²³^ORCID

Affiliation:

1. International Clinical Research Center, Department of Global Health, Schools of Medicine and Public Health, University of Washington , Seattle, Washington , USA

2. Department of Epidemiology, School of Public Health, University of Washington , Seattle, Washington , USA

3. Division of Allergy and Infectious Diseases, Department of Medicine, School of Medicine, University of Washington , Seattle, Washington , USA

4. Department of Immunology, Center for Innate Immunity and Immune Disease, University of Washington , Seattle, Washington , USA

5. Center for Emerging and Re-emerging Infectious Diseases, University of Washington , Seattle, Washington , USA

6. Global Health Labs , Bellevue, Washington , USA

Abstract

Abstract We evaluated the performance of rapid antigen (RAg) and antibody (RAb) microfluidic diagnostics with serial sampling of 71 participants at 6 visits over 2 months following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Rapid tests showed strong agreement with laboratory references (κAg = 81.0%; κAb = 87.8%). RAg showed substantial concordance to both virus growth in culture and PCR positivity 0–5 days since symptom onset (κAg-culture = 60.1% and κAg-PCR = 87.1%). PCR concordance to virus growth in culture was similar (κPCR-culture = 70.0%), although agreement between RAg and culture was better overall (κAg-culture = 45.5% vs κPCR-culture = 10.0%). Rapid antigen and antibody testing by microfluidic immunofluorescence platform are highly accurate for characterization of acute infection.

Funder

Bill and Melinda Gates Foundation

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

Link

https://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiad231/50852894/jiad231.pdf

Reference15 articles.

1. Rapid, point-of-care antigen and molecular-based tests for diagnosis of SARS-CoV-2 infection;Dinnes;Cochrane Database Syst Rev,2020

2. Diagnostic point-of-care tests in resource-limited settings;Drain;Lancet Infect Dis,2014

3. Diagnostics for COVID-19: moving from pandemic response to control;Peeling;Lancet,2022