A Role for Taok2 in Listeria monocytogenes Vacuolar Escape-Reference-Cited by-同舟云学术

A Role for Taok2 in Listeria monocytogenes Vacuolar Escape

Published:2020-06-25 Issue: Volume: Page:
ISSN:0022-1899
Container-title:The Journal of Infectious Diseases
language:en
Short-container-title:

Author:

Quereda Juan J¹²,Morel Camille¹,Lopez-Montero Noelia³⁴,Ziveri Jason⁵⁶,Rolland Steven¹,Grenier Théodore⁷,Aulner Nathalie⁸,Danckaert Anne⁸,Charbit Alain⁵⁶,Enninga Jost³⁴,Cossart Pascale¹,Pizarro-Cerdá Javier⁹

Affiliation:

1. Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France

2. Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos. Facultad de Veterinaria. Universidad Cardenal Herrera-CEU, CEU Universities. Valencia, Spain

3. Unité Dynamique des Interactions Hôte-Pathogène, Institut Pasteur, Paris, France

4. Institut Pasteur, CNRS UMR3691, Paris, France

5. Université Paris Descartes, Sorbonne Paris Cité, Paris, France

6. Institut Necker-Enfants Malades, INSERM U1151-CNRS UMR 8253, Paris, France

7. Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Ecole Normale Supérieure de Lyon, CNRS UMR 5242, Lyon, France

8. Unité de Technologie et de Services Photonic BioImaging/Centre de Ressources et de Recherches Technologiques, Institut Pasteur, Paris, France

9. Unité de Recherche Yersinia, Institut Pasteur, Paris, France

Abstract

Abstract The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.

Funder

Institut Pasteur

Université Paris Diderot

Agence Nationale de la Recherche

Spanish Ministry of Science, Innovation and Universities

European Research Council

Région Ile de France

Groupement d’Intérêt Scientifique Infrastructures en Biologie Santé et Agronomie

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy