Affiliation:
1. China Agricultural University, National Reference Laboratory for Veterinary Drug Residue, Department of Pharmacology and Toxicology, College of Veterinary Medicine, Beijing 100094, People’s Republic of China
Abstract
Abstract
An immunoaffinity chromatographic method was developed for the purification of 9 sulfonamides (sulfamethazine, sulfadimethoxine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamonomethoxine, and sulfapyridine) from chicken tissue (muscle and liver) samples. Two monoclonal antibodies (antisulfamethazine and antisulfamethoxazole) were simultaneously covalently coupled to CNBr-activated Sepharose 4B for the preparation of a re-usable immunoaffinity column. After extraction with methanolwater and purification by immunoaffinity column, the sulfonamides were determined by reversed-phase liquid chromatography and UV detection at 270 nm. The recoveries for each drug at fortification levels of 1050 ng/g ranged from 74.1 to 108.9 with relative standard deviations from 1.9 to 11.5. The limits of detection were 2 ng/g for each drug.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
6 articles.
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