DAPK1 Interacts with the p38 Isoform MAPK14, Preventing Its Nuclear Translocation and Stimulation of Bone Marrow Adipogenesis

Author:

Wang Shan1,Mi Rujia1,Cai Zhaopeng2,Wang Ziming2,Zeng Chenying1,Xie Zhongyu2,Li Jinteng2,Ma Mengjun2,Liu Wenjie2,Su Hongjun3,Cen Shuizhong4,Wu Yanfeng1,Shen Huiyong24ORCID

Affiliation:

1. Center for Biotherapy, Eighth Affiliated Hospital of Sun Yat-Sen University , Shenzhen , People’s Republic of China

2. Department of Orthopedics, Eighth Affiliated Hospital of Sun Yat-Sen University , Shenzhen , People’s Republic of China

3. Center for Biotherapy, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University , Guangzhou , People’s Republic of China

4. Department of Orthopedics, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University , Guangzhou , People’s Republic of China

Abstract

Abstract Bone marrow (BM) adipose tissue (BMAT), a unique adipose depot, plays an important role in diseases such as osteoporosis and bone metastasis. Precise control of mesenchymal stem cell (MSC) differentiation is critical for BMAT formation and regeneration. Here, we show that death associated protein kinase 1 (DAPK1) negatively regulates BM adipogenesis in vitro and in vivo. Prx1creDapk1loxp/loxp mice showed more adipocytes in the femur than Dapk1loxp/loxp mice. Further mechanistic analyses revealed that DAPK1 inhibits p38 mitogen-activated protein kinase (MAPK) signaling in the nucleus by binding the p38 isoform MAPK14, decreasing p38 nuclear activity, which subsequently inhibits BM adipogenesis. The inhibitory effect of DAPK1 against MAPK14 was independent of its kinase activity. In addition, the decreased DAPK1 was observed in the BM-MSCs of ageing mice. Our results reveal a previously undescribed function for DAPK1 in the regulation of adipogenesis and may also reveal the underlying mechanism of BMAT formation in ageing.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

Reference48 articles.

1. Mesenchymal stem cells in health and disease.;Uccelli;Nat Rev Immunol,2008

2. What’s the matter with MAT? Marrow adipose tissue, metabolism, and skeletal health.;Scheller;Ann N Y Acad Sci,2014

3. Marrow adipose tissue: trimming the fat.;Scheller;Trends Endocrinol Metab,2016

4. Traite technique d’histologie.;Ranvier Louis.;Paris,1875

5. The anatomy of the bone marrow: with special reference to the distribution of the red marrow.;Piney;Br Med J,1922

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3