Evaluation of the fermentative capacity of an indigenous Hanseniaspora sp. strain isolated from Lebanese apples for cider production

Author:

AL Daccache Marina12ORCID,Salameh Dominique1,Chamy Laure E L3,Koubaa Mohamed4,Maroun Richard G1ORCID,Vorobiev Eugène2,Louka Nicolas1

Affiliation:

1. Faculté des Sciences, Centre d'Analyses et de Recherche, UR TVA (Technologies de Valorisation Agroalimentaires, Laboratoire CTA, Université Saint-Joseph, Beyrouth 1104 2020, Lebanon

2. Sorbonne University, Université de technologie de Compiègne, ESCOM, EA 4297 TIMR, Centre de recherche Royallieu, CS 60319, 60203 Compiègne CEDEX, France

3. Faculté des Sciences, Centre d'Analyses et de Recherche, UR GPF, Laboratoire BGF, Université Saint-Joseph, Beirut 1104 2020, Lebanon

4. ESCOM, UTC, EA 4297 TIMR, 1 allée du réseau Jean-Marie Buckmaster, 60200 Compiègne, France

Abstract

Abstract The present work studied the fermentative potential and carbon metabolism of an indigenous yeast isolated from Lebanese apples for cider production. The indigenous yeast strain was isolated from a spontaneous fermented juice of the Lebanese apple variety ‘Ace spur’. The sequencing of the Internal Transcribed Spacer (ITS) domain of rRNA identified the isolated yeast strain as a member of the Hanseniaspora genus. These results suggest an intragenomic ITS sequence heterogeneity in the isolated yeast strain specifically in its ITS1 domain. The different investigations on the yeast carbon metabolism revealed that the isolated yeast is ‘Crabtree positive’ and can produce and accumulate ethanol from the first hours of fermentation. Thus, our findings highlight the possibility of using the isolated indigenous Hanseniaspora strain as a sole fermentative agent during cider production.

Funder

Lebanese National Council for Scientific research

Research council of Saint-Joseph University of Beirut

Publisher

Oxford University Press (OUP)

Subject

Genetics,Molecular Biology,Microbiology

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