Gold nanoparticles activate hydrogenase synthesis and improve heterotrophic growth of Ralstonia eutropha H16

Author:

Manutsyan Tatevik1,Blbulyan Syuzanna1,Vassilian Anait2,Semashko Tatiana3,Kirakosyan Gayane4,Gabrielyan Lilit1,Trchounian Karen12ORCID,Poladyan Anna12ORCID

Affiliation:

1. Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University , 0025 Yerevan , Armenia

2. Research Institute of Biology, Biology Faculty, Yerevan State University , 1 A. Manoukian Str., 0025 Yerevan , Armenia

3. Institute of Microbiology, NAS Belarus , 220084 Minsk , Belarus

4. L.A. Orbeli Institute of Physiology NAS RA , 0028 Yerevan , Armenia

Abstract

Abstract Ralstonia eutropha is a facultative chemolithoautotrophic aerobic bacterium that grows using organic substrates or H2 and CO2. Hydrogenases (Hyds) are synthesized under lithoautotrophic, or energy-limited heterotrophic conditions and are used in enzyme fuel cells (EFC) as anodic catalysts. The effects of chemically synthesized gold nanoparticles (Au-NPs) on R. eutropha H16 growth, oxidation-reduction potential (ORP) kinetics, and H2-oxidizing Hyd activity were investigated in this study. Atomic force microscopy showed that thin, plate-shaped Au-NPs were in the nanoscale range with an average size of 5.68 nm. Compared with growth in medium without Au-NPs (control), the presence of Au-NPs stimulated growth, and resulted in a decrease in ORP to negative values. H2-oxidizing activity was not detected in the absence of Au-NPs, but activity was significantly induced (12 U/g CDW) after 24 h of growth with 18 ng/ml, increasing a further 4-fold after 72 h of growth. The results demonstrate that Au-NPs primarily influence the membrane-bound Hyd. In contrast to R. eutropha, Au-NPs had a negligible or negative effect on the growth, Hyd activity, and H2 production of Escherichia coli. The findings of this study offer new perspectives for the production of oxygen-tolerant Hyds and the development of EFCs.

Funder

Ministry of Education

Publisher

Oxford University Press (OUP)

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