Affiliation:
1. Medical Laboratory Technology Department, Koya Technical Institute, Erbil Polytechnic University , Erbil, Kurdistan , Iraq
2. Centre for Epidemiology and Planetary Health, Scotland’s Rural College , Inverness IV2 5NA , UK
3. Department of Genetics and Genome Biology, College of Life Sciences, University of Leicester , University Road, Leicester LE1 7RH , UK
Abstract
Abstract
The environment is a natural reservoir of Clostridioides difficile, and here, we aimed to isolate the pathogen from seven locations in northern Iraq. Four of the sites yielded thirty-one isolates (ten from soils, twenty-one from sediments), which together represent ribotypes (RTs) 001 (five), 010 (five), 011 (two), 035 (two), 091 (eight), and 604 (nine). Twenty-five of the isolates (∼81%) are non-toxigenic, while six (∼19%) encode the toxin A and B genes. The genomes of eleven selected isolates represent six sequence types (STs): ST-3 (two), ST-15 (one), ST-107 (five), ST-137 (one), ST-177 (one), and ST-181 (one). Five novel RT/ST associations: RT011/ST-137, RT035/ST-107, RT091/ST-107, RT604/ST-177, and RT604/ST-181 were identified, and the first three are linked to RTs previously uncharacterized by multilocus sequence typing (MLST). Nine of the genomes belong to Clade 1, and two are closely related to the cryptic C-I clade. Diverse multiple prophages and CRISPR-Cas systems (class 1 subtype I-B1 and class 2 type V CRISPR-Cas systems) with spacers identical to other C. difficile phages and plasmids were detected in the genomes. Our data show the broader diversity that exists within environmental C. difficile strains from a much less studied location and their potential role in the evolution and emergence of new strains.
Funder
MRC New Investigator Research
Publisher
Oxford University Press (OUP)
Subject
Genetics,Molecular Biology,Microbiology