Influence of cyclic di-GMP metabolism to T3SS expression, biofilm formation and symbiosis efficiency in Mesorhizobium japonicum MAFF303099

Author:

Escobar Mariel R1,Lepek Viviana C1,Basile Laura A1ORCID

Affiliation:

1. Instituto de Investigaciones Biotecnológicas “Dr. Rodolfo Ugalde”, Escuela de Bio y Nanotecnologías, Universidad Nacional de San Martín (IIBio-EByN-UNSAM) , CONICET. Av. 25 de Mayo y Francia, San Martín, Buenos Aires , Argentina

Abstract

Abstract A link between the T3SS and inhibition of swimming motility by the transcriptional regulator TtsI in Mesorhizobium japonicum MAFF303099 has been previously reported. Here, we show that mutants in T3SS components display impaired biofilm formation capacity, indicating that a functional T3SS, or at least pili formation, is required for this process. As a first approach to the cdiG regulation network in this bacterium, we started a study of the second messenger cdiG by overexpressing or by deleting some genes encoding cdiG metabolizing enzymes. Overexpression of two putative PDEs as well as deletion of various DGCs led to reduced biofilm formation on glass tubes. Mutation of dgc9509 also affected negatively the nodulation and symbiosis efficiency on Lotus plants, which can be related to the observed reduction in adhesion to plant roots. Results from transcriptional nopX- and ttsI-promoter-lacZ fusions suggested that cdiG negatively regulates T3SS expression in M. japonicum MAFF303099.

Funder

Consejo Nacional de Investigaciones Científicas y Técnicas

Publisher

Oxford University Press (OUP)

Subject

Genetics,Molecular Biology,Microbiology

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