Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study-Reference-Cited by-同舟云学术

Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study

Published:2020-01-07 Issue:6 Volume:71 Page:1554-1557
ISSN:1058-4838
Container-title:Clinical Infectious Diseases
language:en
Short-container-title:

Author:

Kerkhoff Andrew D¹^ORCID,Rutishauser Rachel L²,Miller Steve³,Babik Jennifer M⁴

Affiliation:

1. Division of HIV, Infectious Diseases, and Global Medicine, Zuckerberg San Francisco General Hospital and Trauma Center, Department of Medicine, University of California, San Francisco, California, USA

2. Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA

3. Department of Laboratory Medicine, University of California, San Francisco, California, USA

4. Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, California, USA

Abstract

Abstract We assessed the real-world utility of universal broad-range polymerase chain reaction sequencing for pathogen detection. Among 1062 clinical samples, 107/1062 (10.1%) had a clinically significant, positive result, with substantial variation by specimen type. Clinical management was changed in 44/1062 (4.1%). These data can help maximize utility of this emerging diagnostic.

Funder

National Institute of Allergy and Infectious Diseases

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Microbiology (medical)

Link

http://academic.oup.com/cid/advance-article-pdf/doi/10.1093/cid/ciz1245/33105571/ciz1245.pdf

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