Affiliation:
1. State University of New York (SUNY), Albany, NY, USA
2. Advanced Restoration Technologies, Inc., 9035 North 15th Place, Phoenix, AZ 85020, USA
Abstract
Chronic inflammation of the hair follicle (HF) is considered a contributing factor in the pathogenesis of androgenetic alopecia (AGA). Previously, we clinically tested liposterolic extract ofSerenoa repens(LSESr) and its glycoside,β-sitosterol, in subjects with AGA and showed a highly positive response to treatment. In this study, we sought to determine whether blockade of inflammation using a composition containing LSESr as well as two anti-inflammatory agents (carnitine and thioctic acid) could alter the expression of molecular markers of inflammation in a well-establishedin vitrosystem. Using a well-validated assay representative of HF keratinocytes, specifically, stimulation of cultured human keratinocyte cellsin vitro, we measured changes in gene expression of a spectrum of well-known inflammatory markers. Lipopolysaccharide (LPS) provided an inflammatory stimulus. In particular, we found that the composition effectively suppressed LPS-activated gene expression of chemokines, includingCCL17,CXCL6andLTB(4)associated with pathways involved in inflammation and apoptosis. Our data support the hypothesis that the test compound exhibits anti-inflammatory characteristics in a well-establishedin vitroassay representing HF keratinocyte gene expression. These findings suggest that 5-alpha reductase inhibitors combined with blockade of inflammatory processes could represent a novel two-pronged approach in the treatment of AGA with improved efficacy over current modalities.
Subject
Complementary and alternative medicine
Cited by
20 articles.
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