Affiliation:
1. Department of Zoology, Faculty of Science, University of Mansoura, Mansoura, Egypt
2. Al-Qalam University, Kerkuk, At-Ta'mim, Iraq
3. Department of Surgery, Drew University of Medicine and Science, Los Angeles, CA, USA
Abstract
ABSTRACT
This study examines the ability of arabinoxylan rice bran (MGN-3/Biobran) to enhance the anti-cancer effects of fractionated X-ray irradiation of Ehrlich solid tumor-bearing mice. Swiss albino mice bearing tumors were exposed to the following: (i) Biobran treatment (40 mg/kg/day, intraperitoneal injections) beginning on day 11 post-tumor cell inoculation until day 30; (ii) ionizing radiation (Rad) 2 Gy at three consecutive doses on days 12, 14 and 16; or (iii) Biobran + Rad. Final tumor weight was suppressed by 46% for Biobran, 31% for Rad and 57% for the combined treatment (Biobran + Rad) relative to control untreated mice. Biobran and Rad also arrested the hypodiploid cells in the sub-G1-phase, signifying apoptosis by +102% and +85%, respectively, while the combined treatment induced apoptosis by +123%, with similar results in the degree of DNA fragmentation. Furthermore, Biobran + Rad upregulated the relative gene expression and protein level of p53 and Bax in tumor cells, down-regulated Bcl-2 expression, and increased the Bax/Bcl-2 ratio and caspase-3 activity, with the combined treatment greater than for either treatment alone. Additionally, the combined treatment modulated the decrease in body weight, the increase in liver and spleen weight, and the elevation of liver enzymes aspartate aminotransferase, alanine aminotransferase and gamma-glutamyl transferase to be within normal values. We conclude that Biobran enhances radiation therapy-induced tumor regression by potentiating apoptosis and minimizing toxicities related to radiation therapy, suggesting that Biobran may be useful in human cancer patients undergoing radiotherapy and warranting clinical trials.
Publisher
Oxford University Press (OUP)
Subject
Health, Toxicology and Mutagenesis,Radiology, Nuclear Medicine and imaging,Radiation
Reference46 articles.
1. Basic principles of the molecular biology of cancer II: angiogenesis, invasion and metastasis;Neal;Surgery,2006
2. Global cancer statistics;Jemal;CA Cancer J Clin,2011
3. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008;Ferlay;Int J Cancer,2010
4. The hypoxic cell: A target for selective cancer therapy-Eighteenth Bruce F. Cain Memorial Award lecture;Brown;Cancer Res,1999
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献