In vivo calcium imaging shows that satellite glial cells have increased activity in painful states

Author:

Jager Sara E12ORCID,Goodwin George1,Chisholm Kim I3,Denk Franziska1

Affiliation:

1. Wolfson Centre for Age-related Diseases, King’s College London, Guy’s Campus , London SE1 1UL , UK

2. Molecular Neuropharmacology and Genetics Laboratory, Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen , 2200 Copenhagen , Denmark

3. Pain Centre Versus Arthritis, School of Life Sciences, University of Nottingham , Nottingham NG5 1PB , UK

Abstract

Abstract Satellite glial cells are important for proper neuronal function of primary sensory neurons for which they provide homeostatic support. Most research on satellite glial cell function has been performed with in vitro studies, but recent advances in calcium imaging and transgenic mouse models have enabled this first in vivo study of single-cell satellite glial cell function in mouse models of inflammation and neuropathic pain. We found that in naïve conditions, satellite glial cells do not respond in a time-locked fashion to neuronal firing. In painful inflammatory and neuropathic states, we detected time-locked signals in a subset of satellite glial cells, but only with suprathreshold stimulation of the sciatic nerve. Surprisingly, therefore, we conclude that most calcium signals in satellite glial cells seem to develop at arbitrary intervals not directly linked to neuronal activity patterns. More in line with expectations, our experiments also revealed that the number of active satellite glial cells was increased under conditions of inflammation or nerve injury. This could reflect the increased requirement for homeostatic support across dorsal root ganglion neuron populations, which are more active during such painful states.

Funder

Lundbeck Foundation

IMK Almene Fond

Medical Research Foundation Prize

United Kingdom Research and Innovation Medical Research Council

Anne McLaren Fellowship

University of Nottingham

National Institute for Health and Care Research

Guy’s and St Thomas’ NHS Foundation Trust

NHS

Department of Health and Social Care

UK Research & Innovation

Publisher

Oxford University Press (OUP)

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