Are Predator Smell (TMT)-Induced Behavioral Alterations in Rats Able to Inhibit Seizures?

Author:

Delfino-Pereira Polianna1,Berti Dutra Poliana12,Cortes de Oliveira Jose Antonio2,Casanova Turatti Izabel Cristina3,Fernandes Artur2,Peporine Lopes Norberto3,Garcia-Cairasco Norberto12ORCID

Affiliation:

1. Neurosciences and Behavioral Sciences Departament, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

2. Physiology Departament, Ribeirão Preto School of Medicine, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

3. Physics and Chemistry Departament, Ribeirão Preto School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

Abstract

Abstract We aimed to evaluate the chemical and behavioral effects of 2,5-dihydro-2,4,5-trimethylthiazoline (TMT) after olfactory exposure and to verify their influence in the expression of acute audiogenic seizures in the Wistar Audiogenic Rat (WAR) strain. PROTOCOL 1: TMT gas chromatography was applied to define odor saturation in a chamber to different concentrations, time required for saturation and desaturation, and if saturation was homogeneous. Also, male Adult Wistar rats were exposed to saline (SAL) or to different TMT concentrations and their behaviors were evaluated (neuroethology). PROTOCOL 2: Male adult WARs were exposed for 15 s to SAL or TMT, followed by sound stimulation for 1 min or until tonic–clonic convulsion. Behavioral analysis included latencies (wild running and tonic–clonic convulsion), seizure severity indexes, and neuroethology. Gas chromatography established a saturation homogeneous to different concentrations of TMT, indicating that saturation and desaturation occurred in 30 min. TMT triggered fear-like or aversion-like reactions associated with reduction in motor activity and in grooming behavior, in the 2 highest concentrations. Pure TMT presented anticonvulsant properties, such as less-severe seizure phenotype, as well as a decrease in tonic–clonic convulsion expression. TMT elicited fear-like or aversion-like behaviors in Wistar and WAR and can be utilized in a quantifiable and controllable way. Our results suggested possible antagonism between “fear-related” or “aversion-related” and “seizure-related” networks.

Funder

Fundação de Amparo à Pesquisa do Estado de São Paulo

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Cooperação Interinstitucional de Apoio a Pesquisas sobre o Cérebro-FAPESP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Programa de Excelência da CAPES-Fisiologia

Publisher

Oxford University Press (OUP)

Subject

Behavioral Neuroscience,Physiology (medical),Sensory Systems,Physiology

Reference79 articles.

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5. Freezing to the predator odor 2,4,5 dihydro 2,5 trimethylthiazoline (TMT) is disrupted by olfactory bulb removal but not trigeminal deafferentation;Ayers;Behav Brain Res,2013

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