Comparison of Hydration Index, Percent Hydration, and Trans-Epidermal Water Loss Measurements for Dermal Exposure and Risk Assessment

Abstract

Abstract Skin hydration and the barrier properties of the stratum corneum have been reported to be influential factors in the potential for retention of solid and semi-solid substances on the skin surface. The measurement of these characteristics of the skin, however, remains relatively uncommon in exposure assessments performed by industrial or occupational hygienists, even when the focus of the assessment is exposure to the skin. This study provides measurements of skin hydration using multiple instruments and multiple relevant skin site locations for comparative analysis. Three different measurement metrics, trans-epidermal water loss (TEWL), hydration index (HI), and percent hydration, were measured for 25 healthy volunteers at two different body locations for comparison: the center of the volar forearm, as previously recommended for interindividual comparison of hydration and barrier property measurements, and also the palmar tip of the index finger. The purpose of the comparative measurements was to allow for comparison between other published baseline volar forearm measurements and the palmar skin, which has not often been quantitatively assessed and reported in the literature, but is a relevant skin surface for sampling of the hands. This comparison will allow for consideration of the potential influence of palmar wipe sampling protocols on TEWL or skin hydration, and for the evaluation of the influence of skin hydration and TEWL on measured dermal transfer values. Collectively, the skin hydration levels and barrier properties at these two different measurement locations were found to be statistically significantly different, and as a result it is suggested that they be measured and recorded separately. Both measurement types and locations are likely to be important for the purposes of establishing skin hydration and health. Volar forearm measurements can also be important for understanding the underlying condition and barrier function of the skin, and palmar index finger measurements are necessary to understand the influence of both TEWL and skin hydration on quantitative dermal loading and transfer of solids and semi-volatile materials.